Postmortem Interval Estimation Using Protein Chip Technology Combined with Multivariate Analysis Methods / 法医学杂志

OBJECTIVES@#To estimate postmortem interval (PMI) by analyzing the protein changes in skeletal muscle tissues with the protein chip technology combined with multivariate analysis methods.@*METHODS@#Rats were sacrificed for cervical dislocation and placed at 16 ℃. Water-soluble proteins in skeletal muscles were extracted at 10 time points (0 d, 1 d, 2 d, 3 d, 4 d, 5 d, 6 d, 7 d, 8 d and 9 d) after death. Protein expression profile data with relative molecular mass of 14 000-230 000 were obtained. Principal component analysis (PCA) and orthogonal partial least squares (OPLS) were used for data analysis. Fisher discriminant model and back propagation (BP) neural network model were constructed to classify and preliminarily estimate the PMI. In addition, the protein expression profiles data of human skeletal muscles at different time points after death were collected, and the relationship between them and PMI was analyzed by heat map and cluster analysis.@*RESULTS@#The protein peak of rat skeletal muscle changed with PMI. The result of PCA combined with OPLS discriminant analysis showed statistical significance in groups with different time points (P<0.05) except 6 d, 7 d and 8 d after death. By Fisher discriminant analysis, the accuracy of internal cross-validation was 71.4% and the accuracy of external validation was 66.7%. The BP neural network model classification and preliminary estimation results showed the accuracy of internal cross-validation was 98.2%, and the accuracy of external validation was 95.8%. There was a significant difference in protein expression between 4 d and 25 h after death by the cluster analysis of the human skeletal muscle samples.@*CONCLUSIONS@#The protein chip technology can quickly, accurately and repeatedly obtain water-soluble protein expression profiles in rats' and human skeletal muscles with the relative molecular mass of 14 000-230 000 at different time points postmortem. The establishment of multiple PMI estimation models based on multivariate analysis can provide a new idea and method for PMI estimation.

Identification of serological biomarkers for diagnosis of rheumatoid arthritis using a protein array-based approach / 南方医科大学学报

OBJECTIVE@#To study the cytokine patterns in patients with rheumatoid arthritis (RA) and healthy individuals and identify candidate serum biomarkers for clinical diagnosis of RA.@*METHODS@#This study was conducted among 59 patients diagnosed with RA in our hospital from 2015 to 2019 with 46 age- and gender-matched healthy subjects who received regular physical examinations in our hospital as the control group. Serological autoimmune profiles of 5 RA patients and 5 healthy control subjects were obtained from human cytokine microarrays. We selected 4 differentially expressed cytokines (LIMPII, ROBO3, Periostin and IGFBP-4) and 2 soluble cytokine receptors of interest (2B4 and Tie-2) and examined their serum levels using enzyme-linked immunosorbent assay in 54 RA patients and 41 healthy control subjects. Spearman correlation test was performed to assess the correlation of serum cytokine and soluble receptor expression levels with the clinical features including rheumatoid factor (RF), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), disease activity score (DAS28) and health assessment questionnaire (HAQ). Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic capability of these cytokines.@*RESULTS@#We identified 6 dysregulated cytokines and soluble receptors (2B4, LIMPII, Tie-2, ROBO3, periostin and IGFBP-4) in RA patients (P < 0.01). The serum levels of LIMPII, ROBO3 and periostin were significantly correlated with the disease activity indicators including RF (P < 0.001), CRP (P < 0.001), DAS28 (P < 0.001) and HAQ (P < 0.001) in RA patients. Among the 6 candidate cytokines, 2B4 showed the largest area under the curve (AUC) of 0.861 for RA diagnosis (P < 0.001), followed then by LIMPII, ROBO3, periostin, Tie-2 and IGFBP-4.@*CONCLUSION@#Serum levels of LIMPII, ROBO3 and periostin can be indicative of the disease activity of RA, and serum 2B4, LIMPII, periostin, ROBO3, IGFBP-4 and Tie-2 levels may serve as biomarkers for the diagnosis of RA.

Application of SPR protein chip in screening for imported malaria / 生物工程学报

Imported malaria has become a major risk factor for malaria prevention and control in China. How to screen malaria quickly for people entering China is an urgent problem to be solved. Protein microarrays are widely used in high-throughput screening and diagnosis. In this study, surface plasmon resonance (SPR) technique for malaria detection was established by using the specific adsorption surface treated by polyethylene glycol polymer, and the malaria specific antigen HRP2 was used as capture probe. The optimal concentration of antigen, sensitivity and specificity of detection, as well as anti-interference ability of the chip were analyzed. The SPR protein chip was applied to detect specific antibodies of malignant malaria in serum with the advantage of label-free, instant and fast. Compared with fluorescence quantitative PCR, there were no significant difference in sensitivity and specificity between the two methods. This study lays a foundation for further development of protein microarray for malaria typing identification, and it is conducive to the rapid screening of malaria for people entering.

Screening of differential proteins from follicular fluid of patients with polycystic ovarian syndrome employing protein chip array / 上海交通大学学报（医学版）

Objective: To identify the differential proteins from follicular fluid of patients with polycystic ovarian syndrome (PCOS) employing protein chip array, and provide a theoretical basis for further study of PCOS. Methods: AAH-BLG-507 array was used to test the follicular fluid of 6 PCOS patients with normal body mass index (BMI) and 6 non-PCOS controls with age and BMI-matched. The differential proteins were identified and bioinformatics analysis was carried out by functional annotation analysis, KEGG pathway analysis and protein-protein interaction network analysis. Results: Compared with non-PCOS controls, 25 up-regulated proteins and 49 down-regulated proteins were identified from follicular fluid of patients with PCOS. The classification of differential proteins based on molecular function revealed that they were mainly involved in cytokine activity and chemokine activity. KEGG pathway analysis was performed and pathways associated with cytokine-cytokine receptor interaction and chemokine signaling pathway were significantly enriched. The core proteins filtered by protein-protein interaction network analysis were mainly chemokines and their receptors. Conclusion: Protein chip array can be used to establish the differential expressed protein profile from follicular fluid of patients with PCOS. The cytokinecytokine receptor interaction and chemokine signaling pathway may be involved in the pathogenesis of PCOS.

Application of protein chip and mass spectrometry in dry eye syndromes / 国际眼科杂志(Guoji Yanke Zazhi)

@#Dry eye syndrome is one of the most common ophthalmology diseases. With the improvement of non-invasive tear extraction methods, tear protein analysis becomes a best choice for the diagnosis and evaluation of efficacy of dry eye. Methods that have been used for tear proteomics research include protein chip technology and mass spectrometry. The relationship between the protein in the tear fluid and its concentration and different pathological conditions has important value in the research of dry eye syndromes. This article reviews the latest developments in the field, the application of tear proteomics research and current practical issues,it will provide highlights for the understanding of molecular mechanism of dry eye syndromes and benefits for potential molecular diagnosis of dry eye syndromes in the future.

Changes of cytokines in serum and cerebrospinal fluid of newborns infected with human cytomegalovirus / 中华实用儿科临床杂志

Objective@#To investigate the molecules of cytokine in the serum and cerebrospinal fluid of newborns infected with human cytomegalovirus (HCMV) by using protein chip technology and to analyze the changes of specific cytokine in serum and cerebrospinal fluid caused by HCMV infection, in order to provide a reliable index for predicting nervous system injury caused by HCMV infection.@*Methods@#Serum and cerebrospinal fluid in 4 newborns with HCMV infection and central nervous system injury (HCMV-infected group), and 4 newborns without HCMV infection and central nervous system infection (control group) were collected in Shengjing Hospital of China Medical University from June 2016 to December 2017, and protein chip was used to screen the differentially expressed cytokines in newborns serum and cerebrospinal fluid.The samples were further expanded to collect cerebrospinal fluid from 30 newborns HCMV infection group and 30 newborns in the control group, and the expression of differentially proteins was verified by adopting enzyme linked immunosorbent assay(ELISA) method.@*Results@#The results of protein chip analysis showed that newborns in HCMV infection group, compared with the control group, had 3 differentially expressed cytokines in the cerebrospinal fluid sample: adipocyte complement-related protein of 30 kD(Acrp30), interleukin-1 alpha(IL-1α), and matrix metallo protein-3(MMP3) (all P<0.05). Newborns in the HCMV-infected group, compared with the control group, had no differential cytokine expression in the serum.The results of ELISA showed that expression of Acrp30 was significantly higher in the cerebrospinal fluid of newborns with HCMV infection and central nervous system injury [(39.76±2.01) ng/L vs.(7.75±0.10) ng/L, t=87.09, P<0.001], and MMP3 expression was higher than that of control group [(1.40±2.13) ng/L vs.(0.18±0.45) ng/L, t=3.07, P=0.003], while the expression of IL-1α was significantly lower than that of the control group [(2.36±0.99) ng/L vs.(2.91±0.78) ng/L, t=2.39, P=0.020], and the differences were statistically significant.@*Conclusions@#The changes of cytokine in cerebrospinal fluid of HCMV infected newborn children may provide a reliable index for predicting injury degree of central nervous system in HCMV, and may further assist clinicians to give timely and appropriate treatment to newborns, and further assist clinicians to improve the prognosis for newborns.

Changes of cytokines in serum and cerebrospinal fluid of newborns infected with human cytomegalovirus / 中华实用儿科临床杂志

Objective To investigate the molecules of cytokine in the serum and cerebrospinal fluid of newbo_rns infected with human cytomegalovirus(HCmV)by using protein chip technology and to analyze the changes of spe_cific cytokine in serum and cerebrospinal fluid caused by HCmV infection,in order to provide a reliable index for pre_dicting nervous system injury caused by HCmV infection. Methods Serum and cerebrospinal fluid in 4 newborns with HCmV infection and central nervous system injury(HCmV_infected group),and 4 newborns without HCmV infection and central nervous system infection(control group)were collected in Shengjing Hospital of China medical University from June 2016 to December 2017,and protein chip was used to screen the differentially expressed cytokines in newbo_rns serum and cerebrospinal fluid. The samples were further expanded to collect cerebrospinal fluid from 30 newborns HCmV infection group and 30 newborns in the control group,and the expression of differentially proteins was verified by adopting enzyme linked immunosorbent assay( ELISA)method. Results The results of protein chip analysis showed that newborns in HCmV infection group,compared with the control group,had 3 differentially expressed cytokines in the cerebrospinal fluid sample:adipocyte complement_related protein of 30 kD(Acrp30),interleukin_1 alpha(IL_1α), and matrix metallo protein_3(mmP3)(all P<0. 05). Newborns in the HCmV_infected group,compared with the control group,had no differential cytokine expression in the serum. The results of ELISA showed that expression of Acrp30 was significantly higher in the cerebrospinal fluid of newborns with HCmV infection and central nervous system injury[(39. 76 ± 2. 01)ng/L υs.(7. 75 ± 0. 10)ng/L,t=87. 09,P<0. 001],and mmP3 expression was higher than that of control group[(1. 40 ± 2. 13)ng/L υs.(0. 18 ± 0. 45)ng/L,t=3. 07,P=0. 003],while the expression of IL_1α was significantly lower than that of the control group[(2. 36 ± 0. 99)ng/L υs.(2. 91 ± 0. 78)ng/L,t=2. 39, P=0. 020],and the differences were statistically significant. Conclusions The changes of cytokine in cerebrospinal fluid of HCmV infected newborn children may provide a reliable index for predicting injury degree of central nervous system in HCmV,and may further assist clinicians to give timely and appropriate treatment to newborns,and further as_sist clinicians to improve the prognosis for newborns.

1,2-Dichloroethane-induced apoptosis in human astrocytes through mitochondrial pathway / 中国职业医学

OBJECTIVE: To explore the molecular mechanism underlying 1,2-dichloroethane(1,2-DCE) induced apoptosis by screening differentially expressed proteins in human astrocytes( HAs). METHODS: HAs were cultured in complete medium with 1,2-DCE at various concentrations of 0-80 or 0-40 mmol/L. After 24 hours,apoptosis of HAs was evaluated using flow cytometry and staining with annexin Ⅴ-fluoresce in isothiocyanate and propidium iodide. An AAH-APO-1-2 protein chip was used to screen differentially expressed proteins and quantitative real-time polymease chain reaction(qRT-PCR) was used to verify related differentially expressed genes(DEGs). RESULTS: At 1,2-DCE concentrations of0-80 mmol/L,the total apoptosis rate of HAs increased with 1,2-DCE concentrations in a dose-dependent manner( P <0. 01). Seven different kinds of proteins were screened out by apoptotic protein chip. Among them,the expression of insulin-like growth factor-binding protein( IGFBP)-1,IGFBP-4 and cytochrome C( Cyto C) were up-regulated,while the expression of P27,cysteine aspartic acid specific protease-3( Caspase-3),B-cell lymphoma-2 interacting mediator of cell death( BIM) and BH3 interacting domain death agonist( BID) were down-regulated compared with the control group. The result of DEGs verified by qRT-PCR showed that the expression of mRNA of IGFBP-1,IGFBP-4 and Cyto C at 1,2-DCE concentrations of 40 mmol/L was up-regulated. This result was in consistent with the trend of target expression in the protein chip. The mRNA expression of Caspase-3,BIM and BID was also up-regulated. CONCLUSION: 1,2-DCE induces apoptosis of HAs through mitochondrial pathway.

Preparation and identification of protein chip for detecting components of peach allergen / 基础医学与临床

Objective To prepare a protein chip for the detection of serum antibodies against several peach allergen components, and to provide a rapid and reliable method for clinical diagnosis of peach allergic disease .Methods The coding sequences of six key peach components Pru p 1, Pru p 2, Pru p 3, Pru p 4, Pru p 7 and Pru p G were inserted into pGAPZαA yeast expression vector.Then the vectors were digested by endonuclease Avr Ⅱ and electroporated into yeast SMD1168 for protein expression.After peach proteins were purified, the protein chip was prepared and used to detect the antibodies against peach allergen components in serum samples from 41 suspected patients.The sensitivity and specificity of the protein chip were verified by comparison with ImmunoCAP method .Res ults The protein chip was prepared with these proteins for detecting IgE antibodies in serum samples against these four peach allergen components.The results showed that the sensitivity of the protein chip to Pru p 1, Pru p 3 and Pru p 4 was 40%, 100%and 100%, and the specificity was 100%, 50%and 100%respectively.The total sensitivity and specificity was 86%and 96%respectively.Six serum samples from peach allergy patients were identified Pru p7 antibody positive by the protein chip.Co nclusions The sensitivity and specificity of the protein chip is comparable to ImmunoCAP.It needs less serum and so to be a potential method for the clinical diagnosis of peach allergenic disease after optimization.

Study on the Simultaneously Quantitative Detection for β-Lactoglobulin and Lactoferrin of Cow Milk by Using Protein Chip Technique / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To research a protein chip method which can simultaneously quantitative detect β-Lactoglobulin (β-L) and Lactoferrin (Lf) at one time.</p><p><b>METHODS</b>Protein chip printer was used to print both anti-β-L antibodies and anti-Lf antibodies on each block of protein chip. And then an improved sandwich detection method was applied while the other two detecting antibodies for the two antigens were added in the block after they were mixed. The detection conditions of the quantitative detection for simultaneous measurement of β-L and Lf with protein chip were optimized and evaluated. Based on these detected conditions, two standard curves of the two proteins were simultaneously established on one protein chip. Finally, the new detection method was evaluated by using the analysis of precision and accuracy.</p><p><b>RESULTS</b>By comparison experiment, mouse monoclonal antibodies of the two antigens were chosen as the printing probe. The concentrations of β-L and Lf probes were 0.5 mg/mL and 0.5 mg/mL, respectively, while the titers of detection antibodies both of β-L and Lf were 1:2,000. Intra- and inter-assay variability was between 4.88% and 38.33% for all tests. The regression coefficients of protein chip comparing with ELISA for β-L and Lf were better than 0.734, and both of the two regression coefficients were statistically significant (r = 0.734, t = 2.644, P = 0.038; and r = 0.774, t = 2.998, P = 0.024).</p><p><b>CONCLUSION</b>A protein chip method of simultaneously quantitative detection for β-L and Lf has been established and this method is worthy in further application.</p>

Application of different detection methods in the diagnosis of rheumatoid arthritis / 中国基层医药

Objective To compare the autoantibody detection and enzyme linked immunosorbent assay of protein chip in the diagnosis of rheumatoid arthritis (RA).Methods 81 cases of RA were given autoantibodies protein chip and enzyme linked immunosorbent assay(ELISA),the project included rheumatoid factor(RF)and anti cyclic citrullinated peptide antibody(Anti -CCP),anti nuclear factor(APF),anti keratin antibody(AKA)and anti-nuclear antibody(ANA),recorded the test results,time and cost,the income data were statistically analyzed.Results Patients with RA autoantibody protein chip for detection of CCP,AKA,RF,APF,ANA positive rates were 93.83%, 92.59%,90.12%,95.06%,92.59%,ELISA for the detection of CCP,AKA,RF,APF and ANA positive rates were 95.06%,91.36%,92.59%,91.36%,93.83%,two detection methods had no significant differences(χ2 =1.908, 1.345,1.764,2.246,1.102,all P >0.05);autoantibody detection protein chip required time and cost were respec-tively (1.33 ±0.19)h,(151.24 ±29.83)yuan,which were significantly less than the detection of ELISA (6.81 ± 1.24)h,(899.67 ±121.35)yuan,the differences were statistically significant(t =7.989,8.235,all P <0.05 ). Conclusion The detection of antibody protein chip for RA diagnosis with high accuracy,short time,less cost,help the doctor to diagnose the disease quickly obtain test results.

Application of multi-tumor marker protein chip for tumor screening / 国际检验医学杂志

Objective To study the application of multi‐tumor marker protein chip for early tumor screening and diagnosis . Methods From Nov .2011 to Dec .2015 ,10 736 samples ,including people receiving physical examination and with high risk of canc‐er in Fengcheng Hospital were collected .Twelve tumor markers in serum(AFP ,CEA ,NSE ,CA125 ,CA153 ,CA242 ,CA199 ,PSA ,f‐PSA ,FER ,β‐HCG and HGH)were measured by multi‐tumor markers protein chip detective system ,and the results were analyzed . Results We found out 967 samples with positive markers in the 10 736 samples .Of which ,496 were male and 471 were female ,the positive rate were 4 .62% and 4 .39% respectively .Totally 473 were diagnosed with tumor confirming by clinical pathology ,postive diagnosis rate was 48 .91% .Conclusion The multi‐tumor marker protein chip (C12 system) can detect multiple tumor markers simultaneously to improve screening process and achieve rapid detection ,w hich has higher positive detectiong rate and clinical value on diagnosing malignant tumor in early stage .

Clinical application value of C-12 protein chip detection system / 国际检验医学杂志

Objective To study the value of the multiple tumor markers protein chip system (C‐12) in the clinical application . Methods The levels of 12 kinds of tumor markers were detected in 5 000 healthy people (healthy physical examination group) ,4 500 patients with benign tumor and inflammation (benign disease group) and 5 500 patients with malignant tumors (malignant tumor group) .At the same time 1 369 negative specimens in the malignant tumor group were performed the comparative analysis with the electrochemiluminescence method by adopting the fully automatic chemiluminescence immune analyzer .Results The posi‐tive rate in the malignant tumor group by using C‐12 was 75 .11% ,which was significantly higher than 20 .31% in the benign dis‐ease group and 4 .34% in the healthy physical examination group ,the difference between the two groups in pair had statistical sig‐nificance(P< 0 .05) .The sensitivity of C‐12 for detecting malignant tumor was 75 .11% ,the specificity was 95 .66% ,and the posi‐tive rate of joint detection was significantly higher than that of single marker ,the difference between them was statistically signifi‐cant (P< 0 .05) .Conclusion The 12 tumor markers protein chip joint detection method has certain reference value for the diagnosis in the patients with tumor .Detecting multiple tumor markers can obviously increase the diagnosis sensitivity of malignant tumors , can be used in the prognosis and the curative effect observation ,at the same time can also be used as one of screening measures for early tumor among asymptomatic population .

The clinic value of multi-tumor marker protein chip in breast cancer with neoadjuvant chemotherapy / 重庆医学

Objective To observe the evaluation of multi‐tumor marker protein chip in breast cancer with neoadjuvant chemo‐therapy ,and to find high saitivity tumor makers ,then evaluate the clinical significance .Methods Forty health examination persons (control group) and sixty‐four breast cancer patients(experimental group) were accepted with multi‐tumor marker protein chip in‐spection ,then we evaluated the markers of diagnosis .The experimental group patients were accepted four cycles preoperative chem‐otherapy with TAC plan ,and tested multi‐tumor marker protein chip three weeks after therapy .Then we evaluated the clinical sig‐nificance of the markers above‐mentioned .Results The positive rate of CA153 ,CA125 ,CEA and FT was 32% ,27% ,21% and 19% in the experimental group ,it indicated significant change compared with the control group (P<0 .05) .And it indicated signifi‐cant reducing change of CA153 ,CA125 and CEA in the experimental group (P<0 .05) after therapy .The sensitivity would be in‐creased when the four markers were tested combined .Conclusion CA153 ,CA125 ,CEA and FT could be used as assistance diagno‐sis index ;CA153 ,CA125and CEA could keep consistence to the curative effect of neoadjuvant chemotherapy and have clinical value .

Screening and analysis of Chinese herbs with liver channel tropism acting on human liver cancer cell differential expressed protein tyrosine kinase / 中国中西医结合急救杂志

Objective To screen the Chinese drugs with liver channel tropism acting on the characteristic differential expressed protein of human liver cancer cell protein tyrosine kinase (PTKs) system by protein chip technology, and to analyze the difference in different Chinese drugs with liver channel tropism in relation to PTKs regulation. Methods Forty BALB/C nude mice were chosen; a piece of subcutaneous tumor mass was implanted into the left lobe of liver parenchyma to reduplicate the orthotopically implanted tumor models. After modeling for 10 days, the tumorigenicity was confirmed, and all the nude mice models were divided into four groups; different Chinese medicine extracts were injected intra-peritoneally into corresponding treatment groups respectively, and the methods of treatment in the 4 groups were as follows: In liver channel tropism drug pair of Huayu Xiaozhen group, rhizoma sparganii and curcuma zedoary with dosage containing crude drug 4.5 g·kg-1·d-1 was given, in liver channel tropism drug pair of Gongdu Sanjie group, the extract of centipede and scorpion with dosage containing crude drug 0.3 g·kg-1·d-1 was intra-peritoneally injected, in spleen channel tropism drug pair control group, astragalus and rhizoma atractylodis macrocephalae with dosage containing crude drug 6.3 g·kg-1·d-1 was given, and in the model group, equal amount of 0.9% normal saline was intra-peritoneally injected. After the drug treatment for 3 weeks, the mice were sacrificed and the liver cancer tissue was obtained; after its total protein was extracted, protein chip technology was applied to screen the Chinese drug pair with liver channel tropism acting on differential expressed PTKs of human liver cancer cells, and the results were analyzed by cluster analysis.Results After the end of the experiment, there were 6 animals in Huayu Xiaozhen drug pair group, 5 in Gongdu Sanjie drug pair group, 5 in control drug pair group and 7 in model group. The protein chip screening results showed that the adjusting fold greater > 1.50 or < 0.67 was defined as the difference with statistical significance. Compared with model group, there were 42 up-regulated expressions of PTKs in Huayu Xiaozhen drug pair group, including 29 receptor tyrosine kinases (RTKs) and 13 non-receptor protein tyrosine kinases (nrPTKs) of which the erythropoietin having adjusting fold over 5.0 produced liver cell receptor B1 (EphB1), epidermal growth factor receptor (ErbB2, ErbB4) etc. 3 RTKs; there were 7 RTKs with adjusting fold 3.0 - 5.0 including EphA1, EphA3, EphA7, fibroblast growth factor receptor 2-α (FGFR2-α), hepatocyte growth factor receptor (HGFR), macrophage colony-stimulating factor receptor (M-CSFR) and vascular endothelial growth factor receptor 2 (VEGFR2), and 2 nrPTKs with adjusting fold 3.0 - 5.0 were non-receptor tyrosine kinase BMX (BMX) and Janus kinase 1 (JAK1). In the Gongdu sanjie drug pair group, there were 23 up-regulated expressions: 15 RTKs and 8 nrPTKs, and 6 down-regulated expressions; the RTKs with adjusting fold 3.0 - 5.0 were ErbB4, M-CSFR and 1 nrPTKs that was megakaryocyte-associated tyrosine kinase (MATK). In the control drug pair group, there were 28 up-regulated and 8 down-regulated expressions. The results of cluster analysis showed that in Huayu Xiaozhen drug pair group, there were 17 differential expressed PTKs in accord with the screen criteria of which 9 were RTKs [receptor tyrosine kinase-like orphan receptor 2 (ROR2), stem cell factor receptor (SCFR), anaplasia lymphoma kinase (ALK), platelet-derived growth factor receptor β (PDGFR-β), insulin-like growth factor-IR receptor (IGF-IR), ErbB2, ErbB3, EphB1 and EphA2], 1 nrPTKs [fps/fes related tyrosine kinase (FER)] and 7 PTKs 3 RTKs (M-CSFR, FGFR2-α, EphA3) and 4 nrPTKs [acetate kinase 1 (ACK1), bruton tyrosine kinase (Btk), non-receptor tyrosine kinase ABL1 (ABL1) and BMX]. In Gongdu Sanjie drug pair group, there were 7 differential expressed PTKs in accord with the screen criteria 5 RTKs (M-CSFR, FGFR1, ROR2, EphB1, ErbB2) and 2 nrPTKs [src-related kinase lacking C-terminal regulation and N-terminal myristylation sites (SRMS), FER]. Conclusions The drug pair of centipede and scorpion with Gongdu Sanjie action possesses a more effective anti-HCC role than the drug pair of rhizoma sparganii and curcuma zedoary with Huayu Xiaozhen action, the mechanism is possibly via the regulation of PTKs signal pathway. The liver channel tropism drug pair of rhizoma sparganii and curcuma with action of promoting blood circulation and removing blood stasis possibly has an independent anti-HCC effective pathway outside the PTKs signal system.

Stem cells protein microarray analysis after chicken egg-white extract induced cells / 中国免疫学杂志

Objective:To analyze the chicken egg-white extracts were co-cultured with cells whether elevated stem cells protein,whether the cells transformation into stem cell.Methods:Four kinds of cells,making a common culture,a 50% chicken egg-white extract co-cultured for 3 days,cells were collected and frozen at -80 degrees,sending the company to do stem cell protein microarray.Results:C57-BMSC has three proteins occurred statistically significant change , TS-UC-MSC has one proteins occurred a statistically significant change ,293T has one protein occurred a statistically significant change ,and 293T-GFP has one protein occurred a statistically significant change.Conclusion:50% chicken egg-white extract co-cultured cells,the cells occurred the phenomenon of transformation into stem cells.

Comparison of TB-IGRA and Protein Chip in the Diagnosis of Mycobacterium Tuberculosis / 现代检验医学杂志

Objective To evaluate the advantages of TB-IGRA and protein chip to detect the Mycobacterium tuberculosis. Methods From October 2013 to March 2014,collected 78 cases of clinical diagnosis of tuberculosis and normal control’s pe-ripheral blood specimens,used TB-IGRA kits and Mycobacteriumtuberculosis IgG kit(protein chip)to detected respectively. The results were analyzed and compared.Results The sensitivity of protein chip and TB-IGRA in the detection of Mycobac-teriumtuberculosis were 34.5% and 89.7% respectively,which was statistically significant (χ2=26.95,P 0.05).The positive rate of TB-IGRA and Protein chip in tuberculosis were 90.5% and 42.9%.The positive rate of TB-IGRA and Protein chipin extrapulmonary tuberculosis were 89.20% and 29.7% respectively.Conclusion Compared TB-IGRA and protein chip,either diagnose tuberculosis or extrapulmonary tuberculosis has highly positive rate and sensitivity, TB-IGRA can be widely used in the early screening of tuberculosis.

Clinical utility of tuberculosis protein chip in diagnosis of tuberculosis / 中国感染与化疗杂志

Objective To explore the potential value of tuberculosis protein chip for clinical diagnosis of tuberculosis.Methods The antibody level of tuberculosis protein ESAT-6,CFP10,16 KD,38 KD and LAM was determined in 4 093 patients,inclu-ding 441 tuberculosis and 3 652 non-tuberculosis cases by protein chip.Results The tuberculosis antibody was positive in 297 of the 441 tuberculosis cases and 647 of the 3 652 non-tuberculosis cases.Tuberculosis protein chip provided a sensitivity of 67.35% and specificity of 82.28% in the diagnosis of tuberculosis.Conclusions Tuberculosis protein chip test is a quick,easy and effective method for identifying potential tuberculosis patients with good specificity.

Diagnostic value of pulmonary tuberculosis to detect the anti mycobacterium tuberculosis antibody in Serum by the mycobacterium tuberculosis protein Chip and the M.tuberculosis Antibody Colloidal Gold Diagnostic Kit / 中国医师进修杂志

Objective To evaluate the Diagnostic value of pulmonary tuberculosis to detecting the anti Mycobacterium tuberculosis antibody with serum samples by the mycobacterium tuberculosis protein Chip and the M.tuberculosis Antibody Colloidal Gold Diagnostic Kit.Methods Application of mycobacterium tuberculosis protein Chip and the M.tuberculosis Antibody Colloidal Gold Diagnostic Kit to detecting the anti Mycobacterium tuberculosis antibody with serum samples,These serum samples are from 110 cases of tuberculosis patients,60 cases of lung disease and 60 cases of healthy people.Results Mycobacterium tuberculosis protein chip sensitivity was 73.6％ (81/110),the specificity was 93.3％ (112/120),the diagnostic kit for detection of Mycobacterium tuberculosis antibody colloidal gold assay sensitivity was 64.5％ (71/110)and a specificity of 91.7％ (110/120),both the sensitivity and specificity compared to no significant difference (P ＞ 0.05).Conclusions The Tuberculosis protein chip and the M.tuberculosis Antibody Colloidal Gold Diagnostic Kit to detect serum Mycobacterium tuberculosis antibodies for diagnosis of TB has a high sensitivity and specificity.Both can be used for the auxiliary diagnosis of tuberculosis.

Multiple tumor marker chip for detection of serum carcinoembryonic antigen in various malignant tumors: Results and diagnostic value analysis / 第二军医大学学报

Objective To analyze the expression of carcinoembryonic antigen (CEA) in various populations and the simultaneous expression of other markers using multiple tumor marker protein chip, and to discuss the possible clinical relevance. Methods A total of 25 076 profiles of multiple tumor marker protein chip were collected in our hospital for analysis. The elevation of CEA in various populations and commonly-seen tumors was analyzed, and the combined elevation of CEA and other markers was also analyzed in tumors. Results Elevation of CEA in patients with malignant tumors was significantly more than those in patients with benign lesions and normal controls (P<0. 01), with the highest positive rate of CEA seen in the colorectal cancer (41. 85%), followed by pancreatic cancer (37. 97%) and lung carcinoma (37. 16%). CEA was always accompanied by other markers in tumor patients, with the mostly seen elevated marker being CA125, followed by CA19-9 and CA242. The CEA/CA125 was often seen in pancreatic cancer (74. 26%), ovarian cancer (69. 57%), hepatocellular carcinoma (62.13%), and lung cancer (51. 68%); CEA/CA19-9 was often seen in pancreatic cancer (77.23%) and hepatocellular carcinoma (72.34%); CEA/CA242 was often seen in pancreatic cancer(77. 23%) and colorectal cancer(57. 61%); CEA + CA19-9 + CA242 was usually seen in pancreatic cancer(76. 24%), ovarian cancer(52. 17%), and colorectal cancer (51. 32%); and CEA+CA19-9 + CA242 + CA125 was mostly seen in pancreatic cancer(61. 39%). Conclusion CEA is widely expressed in malignant tumors, but it is not specific for malignant tumors. Single elevation of CEA has high value for diagnosis of colorectal cancer, pancreatic cancer, and lung carcinoma. CEA combined with CA125, CA19-9 or CA242 can help to improve the positive rate for diagnosis of pancreatic cancer, ovarian cancer and colorectal cancer.